pGL4.11[luc2P]
- 產(chǎn)品貨號(hào)GS-1728
- 銷售價(jià)格¥ 680
- 包裝規(guī)格2μg質(zhì)?;?.5ml甘油菌
- 載體抗性Ampicillin (氨芐青霉素)
- 載體大小4370 bp
- 備注說(shuō)明Promoterless vector encoding destabilized luciferase for measuring the activity of promoter and enhancer sequences.
The pGL4.11[luc2P] Vector(a–d)encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.11[luc2P] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
0001 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
0061 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT
0121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC
0181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC
0241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG
0301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG
0361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC
0421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC
0481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA
0541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC
0601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC
0661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC
0721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC
0781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC
0841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC
0901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG
0961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG
1741 ATCGCCGTGA ATTCTCACGG CTTCCCTCCC GAGGTGGAGG AGCAGGCCGC CGGCACCCTG
1801 CCCATGAGCT GCGCCCAGGA GAGCGGCATG GATAGACACC CTGCTGCTTG CGCCAGCGCC
1861 AGGATCAACG TCTAAGGCCG CGACTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA
1921 TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT
1981 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC
2041 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG
2101 TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTCGACCG
2161 ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC
2221 GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG
2281 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
2341 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
2401 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
2461 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
2521 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
2581 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
2641 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
2701 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
2761 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
2821 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
2881 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
2941 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
3001 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
3061 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
3121 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
3181 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGCGGCCGCA AATGCTAAAC
3241 CACTGCAGTG GTTACCAGTG CTTGATCAGT GAGGCACCGA TCTCAGCGAT CTGCCTATTT
3301 CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA
3361 CCATCAGGCC CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC CCCCGATTTG
3421 TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA GTGGTCCTGC TACTTTGTCC
3481 GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG TAAGAAGTTC GCCAGTGAGT
3541 AGTTTCCGAA GAGTTGTGGC CATTGCTACT GGCATCGTGG TATCACGCTC GTCGTTCGGT
3601 ATGGCTTCGT TCAACTCTGG TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA
3661 TGAAGAAATG CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCG
3721 GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC TTACCGTCAT GCCATCCGTA
3781 AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT TTTGTGAGTA GTGTATACGG
3841 CGACCAAGCT GCTCTTGCCC GGCGTCTATA CGGGACAACA CCGCGCCACA TAGCAGTACT
3901 TTGAAAGTGC TCATCATCGG GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG
3961 CTATTGAGAT CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC AGCATCTTTT
4021 ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC AAAATGCCGC AAAGAAGGGA
4081 ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC TTTTTCAATA TTATTGAAGC
4141 ATTTATCAGG GTTACTAGTA CGTCTCTCAA GGATAAGTAA GTAATATTAA GGTACGGGAG
4201 GTATTGGACA GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT
4261 GTGTGAATCG ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT
4321 AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT